What is the role of PCR amplification in validating genome edits?

• A. It amplifies the target locus to allow sequencing for confirmation
• B. It digests the genome
• C. It mutates the genome
• D. It labels proteins

Answer: (A)

PCR amplification targets the region around the genome edit, producing many copies of that exact DNA segment. This makes it feasible to sequence the locus and read out the precise changes that were introduced, confirming that the intended modification is present and allowing detection of any unintended alterations or mosaicism. By designing primers on either side of the edit, you isolate the specific locus and generate an amplicon that can be analyzed by Sanger or next‑generation sequencing for clear confirmation. This is not about cutting the genome (digestion), introducing new mutations (mutating), or labeling proteins; those tasks require different methods. PCR simply provides enough, specific DNA material to verify the edit through sequencing.