Name the enzyme used in Stage 1 of fragmenting DNA?

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Multiple Choice

Name the enzyme used in Stage 1 of fragmenting DNA?

Explanation:
Stage 1 of fragmenting DNA hinges on cutting the DNA at specific sites, which is done by restriction endonuclease. This enzyme recognizes defined DNA sequences and makes double-strand breaks, producing fragments that can have blunt or sticky ends depending on the enzyme. This initial digestion is foundational for many downstream techniques like cloning or gel analysis. Other enzymes play different roles: DNA polymerase synthesizes new DNA, not fragments it; ligase joins separate DNA fragments together; helicase unwinds the DNA duplex during replication, not fragmentation.

Stage 1 of fragmenting DNA hinges on cutting the DNA at specific sites, which is done by restriction endonuclease. This enzyme recognizes defined DNA sequences and makes double-strand breaks, producing fragments that can have blunt or sticky ends depending on the enzyme. This initial digestion is foundational for many downstream techniques like cloning or gel analysis. Other enzymes play different roles: DNA polymerase synthesizes new DNA, not fragments it; ligase joins separate DNA fragments together; helicase unwinds the DNA duplex during replication, not fragmentation.

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